microscopic examination of plants
To observe plant cells, it is necessary to make thin sections of their tissues. Depending on the purpose of the study, the type of incision varies in different tissues (for example, in the case of cylindrical limbs such as roots and stems, incisions are made longitudinally or transversely). Then, the best cuts are selected for painting and the next steps are performed on them. Colors help differentiate distinct textures to distinguish cells and their appendages. Plant tissue staining is done in two simple and double ways. In simple staining, the goal is to study the condition of a type of tissue. For this reason, a dye is used that specifically dyes the texture. In double staining, the two main categories of textures (wood and cellulose textures) are distinguished from each other, for which two colors are used. One color dyes cellulose and the other dyes wood, cork or couture. After staining, the sections are ready for microscopic study.
Microscopic examination of animals:
Animal cells are smaller than plant cells. Therefore, the thickness of the layers that are prepared for examination is less than plant tissues, so it is necessary to cut them with the help of special devices called microtomes. Also, due to the softness of the animal tissues, it is necessary to prepare a paraffin tissue mold before cutting. To do this, the tissue is placed in a special container and the container is filled with molten paraffin. After closing the paraffin, a microtome device is used to prepare a cut with a certain thickness.
